Nucleotide sequence of a parathyroid hormone-related peptide expressed by the 10 day chicken embryo.

A fragment from the coding sequence of a human parathyroid hormone-related peptide (PTH-RP) cDNA (1-3) was used to isolate from a lambda gt-11 cDNA library prepared from 10 day old chicken embryo poly A + RNA (Clontech Laboratories Inc.) a 1411 base pair cDNA pcPLPl (bases 16-1436) which encodes the chicken homolog. In the sequence below, the underlined nucleotides (bases 1 —25) were determined from chicken PTHRP genomic DNA isolated from a chicken lambda EMBL phage library (Clontech). The open reading frame of 176 amino acids is verified by the presence of a 5' in-frame termination codon (bases 1 3 ) . In analogy to the human (1 3 ) and rat (4, 5) PTHRP molecules, the arrow marks the predicted site of cleavage of the putative 37 amino acid preproleader sequence from the 139 residue PTHRP. Unique to the chicken PTH-RP are two potential sites for N-linked glycosylation (an (o) is found below the middle residue of the triplet).